Design of a gold nanoprobe for the detection of Pseudomonas aeruginosa elastase gene (lasB)
نویسندگان
چکیده
منابع مشابه
Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region.
The enzyme elastase is an important virulence factor of the opportunistic human pathogen Pseudomonas aeruginosa. Previous studies have shown that expression of the P. aeruginosa elastase gene (lasB) requires both an activator protein, LasR, and an N-acylhomoserine lactone compound termed Pseudomonas autoinducer (PAI). In this study, we analyzed the lasB promoter region to learn more about lasB ...
متن کاملinvestigating the feasibility of a proposed model for geometric design of deployable arch structures
deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...
A second operator is involved in Pseudomonas aeruginosa elastase (lasB) activation.
Pseudomonas aeruginosa LasB elastase gene (lasB) transcription is controlled by the two-component quorum-sensing system of LasR, and the autoinducer, 3OC(12)-HSL (N-3-[oxododecanoyl]homoserine lactone). LasR and 3OC(12)-HSL-mediated lasB activation requires a functional operator sequence (OP1) in the lasB promoter region. Optimal activation of lasB, however, requires a second sequence of 70% id...
متن کاملElastase LasB of Pseudomonas aeruginosa promotes biofilm formation partly through rhamnolipid-mediated regulation.
Elastase LasB, an important extracellular virulence factor, is shown to play an important role in the pathogenicity of Pseudomonas aeruginosa during host infection. However, the role of LasB in the life cycle of P. aeruginosa is not completely understood. This report focuses on the impact of LasB on biofilm formation of P. aeruginosa PAO1. Here, we reported that the lasB deletion mutant (ΔlasB)...
متن کاملPseudomonas aeruginosa Elastase
The elastase produced by Pseudomonas aerughosa is probably responsible for the tissue destruction observed during pulmonary and corneal infections by this pathogen. We have synthesized a new substrate, AbzAla-Gly-Leu-Ala-Nba, for P. aeruginosa elastase. Cleavage of the peptide by elastase at pH 7.2 results in 6to 7-fold increase in fluorescence (h,,, 340 nm; X, 415 nm). A sensitive rate assay u...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RSC Advances
سال: 2020
ISSN: 2046-2069
DOI: 10.1039/d0ra00848f